Mycoplasma remains one of the most persistent and damaging contaminants in laboratory cell cultures. Often invisible under a microscope, it silently alters cellular behavior, interferes with gene expression, and compromises experimental reproducibility. To keep your research on track, early detection and fast elimination are crucial.
That’s where AffiGEN’s 3-step mycoplasma control system comes in.
🔍 Step 1 : Early Detection with AffiZYME® MycoVisual Kit
The first step in safeguarding your lab is routine monitoring. The AffiZYME® MycoVisual Kit offers a fast and visual detection method using LAMP technology. It detects mycoplasma DNA directly from culture supernatant without the need for complex equipment.
Why labs rely on it :
- Colorimetric results in 60 minutes
- Visual readout—blue = clean, purple = contaminated
- Closed-tube design prevents cross-contamination
- Ideal for weekly routine checks
🧪 A must-have for every lab practicing cell culture quality control.
🧬 Step 2 : Confirm with High-Sensitivity AffiPCR® MycoDetect Kit
For definitive results, confirm visual positives using quantitative real-time PCR. The AffiPCR® MycoDetect Kit delivers ultra-sensitive, highly specific mycoplasma detection from as few as 10 genome copies.
📊 Why It’s Trusted:
- Real-time detection in under 2 hours
- Works directly from supernatant—no DNA extraction
- Includes internal positive and negative controls
- Validated for over 60 mycoplasma species
This kit is perfect for regulated environments and research requiring high assay confidence.
🔗 Order the AffiPCR® MycoDetect Kit
💊 Step 3 : Safe Elimination with AffiCLEAN® MycoX Removal Mix
After confirmation, your next goal is clean-up. The AffiCLEAN® MycoX Removal Mix offers an effective, non-cytotoxic removal solution that works inside the cell culture medium to eliminate contamination without damaging your precious cells.
Highlights:
- Removes 70+ known mycoplasma species
- Gentle on mammalian cell lines
- 1:1000 dilution—no media change required
- Decontaminates cultures in 2 to 3 weeks
🧼 Keep your valuable cultures alive and uncontaminated.
👉 Order AffiCLEAN® MycoX Removal Mix
🧭 A Smarter, Faster, Safer Mycoplasma Control System
What sets AffiGEN apart is the integration of screening, confirmation, and elimination into a single, lab-tested workflow. No more guesswork, delayed results, or ineffective antibiotics.
| Step | Product | Result |
|---|---|---|
| 1 | AffiZYME® MycoVisual Kit HERE | Rapid LAMP detection |
| 2 | AffiPCR® MycoDetect Kit HERE | Accurate qPCR confirmation |
| 3 | AffiCLEAN® MycoX Mix HERE | Complete mycoplasma removal |
📊 Why It Matters : Protect Your Science
Contaminated cell cultures lead to:
- Altered experimental outcomes.
- Wasted reagents and time.
- Invalid publications.
- Risk to therapeutic research.
Implementing a robust control strategy not only protects your data but also your lab's reputation.
🧪 Ready to Clean Your Cultures ?
Eliminate hidden contamination and restore confidence in your results. Use the AffiGEN trio:
- Detect – AffiZYME® MycoVisual Kit
- Confirm – AffiPCR® MycoDetect Kit
- Eliminate – AffiCLEAN® MycoX Removal Mix
📦 Fast shipping | 🔬 Expert support | 🧫 Trusted by scientists worldwide