Myristoylated Protein Assay Kit
(FACS & Microscopy)
Green Fluorescence
Unlock precise, in‑cell detection of protein myristoylation with our all‑in‑one Myristoylation Assay Kit—optimized for both flow cytometry (FACS) and fluorescence microscopy. Featuring green fluorescence click‑chemistry, this kit empowers researchers to profile N‑myristoyltransferase (NMT) activity, track membrane‑targeted proteins, and screen inhibitors with publication‑ready results.
Overview
The Myristoylated Protein Assay Kit leverages alkynyl‑myristic acid labeling and a copper‑catalyzed click reaction to attach a fluorescent Azide‑FAM tag onto newly myristoylated proteins—no radioactivity required. Complete your green fluorescence myristoylation assay in under 6 hours.
- Protein Myristoylation Assay Kit | Myristoylation Detection Kit
- Click‑Chemistry Lipidation Assay | Green Fluorescence Myristoylation
- FACS Myristoylation Kit | Fluorescent Myristoylated Protein Assay
- In‑Cell NMT Activity Assay | Alkynyl‑Myristic Acid Labeling
- High Sensitivity & Specificity: > 95 % labeling efficiency, RFI > 5 000 AU
- Dual Detection Modes: Flow cytometry & fluorescence microscopy (FITC/GFP)
- Broad Sample Compatibility: Mammalian cells, yeast, bacteria, viral particles
- Streamlined Workflow: Four simple steps, < 6 hours total
Protocol: Four‑Step Click‑Chemistry Myristoylation Assay
Metabolic Labeling : Incubate cells with 50 µM alkynyl‑myristic acid in complete medium (4 h, 37 °C, 5 % CO₂).
Fixation & Permeabilization : Fix in 4 % PFA (10 min), permeabilize with 0.1 % Triton X‑100 (5 min), wash 3× PBS.
Click Reaction : Add Azide‑FAM (5 µM), CuSO₄ (1 mM), TCEP (1 mM), TBTA (100 µM); incubate 30 min RT, dark.
Detection : Analyze by FACS (FITC channel) or image by fluorescence microscopy (GFP filter).
Kit Contents
Alkynyl‑Myristic Acid Reagent for metabolic labeling
Fixation & Permeabilization Buffers
Azide‑FAM + Copper Click‑Chemistry Cocktail
Wash & Resuspension Buffers
Step‑by‑Step Protocol & Troubleshooting Guide
